Sustainable Biotech, Inc.

Fermentation Experience

Host Cells and Products

• Recombinant protein expression systems, including E. coli, P. pastoris, S. cerevisiae, B. subtilis
• Mammalian tissue culture, including murine and bovine hybridoma cell lines and human cell lines
• Bacterial, fungal and yeast systems, including B. licheniformis, C. botulinum, S. erythraea, A. orientalis, S. spectabilis and many others
• Products include vaccines, antibiotics, monoclonal antibodies, thrombolytic agents, therapeutic neurotoxins, alpha and fungal amylases

Upstream Fermentation Processes

• Fermentor Volumes from 10 L to 150,000 L
• System Capacities over 50 Million Gallons / Year
• Anaerobic and Aerobic Systems (up to 2 vvm)
• Methanol Feed Strategies and Systems
• Low-Flow Micronutrient Feed Systems
• Gas Dispersion, Solids Suspension, Shear
• Process Monitoring and Control
• OUR and kLa Determination
• Mixing Time Optimization
• Sparge Nozzle Location
• Impellor Configurations

Upstream Recovery Processes

• Lysis and Cell Permeabilization Systems
• Whole Cell Recovery
• Microfilters and Ultrafilters
• Belt Filters
• Filter Presses
• Rotary Drum Filters
• Decanters and Centrifuges
• Homogenizers
• Depth Filters
• Lysis Optimization of pH and Heat Labile Products
• ID and Remediation of Solid Formation in Vaccine
• Polyacrylamide Flocculant Systems
• On-Demand Demulsifier Feed Systems
• Cell Permeabilization Optimization

Downstream Purification Processes

• Conversion Steps (e.g. Disulfide Conversion)
• Liquid - Liquid Extraction and Crystallization
• Amyl Acetate, Methylene Chloride
• Chromatography
• Hydrophobic Interaction Chromatography
• Hydrophobic Charge Induction Chromatography
• Cation Exchange Chromatography
• Anion Exchange Chromatography

Downstream Fill/Finish Processes

• Diafiltration and Ultrafiltration
• Aseptic and Terminally Sterilized Fill / Finish
• Lyophilization and Small Volume Liquid Fill
• Drying, Milling and Blending of Solid Dosage

Fermentation Highlights

We love this stuff!